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The stability of 2 H. bovis antigens prepared by different methods was examined by the ELISA. It was found that the activity of H. bovis secretory antigen kept at - 18°C and -70° C and full antigen stored at - 18°C was significantly decreased as early as 6 months after storage. Lyophilized, secretory H. bovis antigen showed constant, high activity and may be used for one year at least.
Thirty-seven cows affected with clinical mastitis (51 quarters) were injected iv with a single 0.02 mg/kg dose of Lydium-KLP. Fifteen cows were injected with both Lydium-KLP at the same dose given twice at a 24-hour interval and intramammary infusion of the agent (0.2 mg per quarter). It was found that the efficacy of the agent depended on the number of somatic cells in the mastitic milk and on their...
The aim of the study was to adapt PCR for detection of BHV 1 after in vitro multiplication. Primers were synthesised for a fragment of the gIV glycoprotein gene of the reference Cooper strain of BHV 1. Polish BHV 1 isolates obtained in the 1970s from bull semen were examined. A positive amplification occurred for the strains belonging to subtype BHV 1.2 and subtype BHV1.1. No amplification has occurred...
The immunoprophylactic properties of Propionibacterium avidum KP-40, a potent stymulator of the macrophage-monocyte system and inducer of endogenous interferon, were tested in newborn calves. A single subcutaneous injection of the immunomodulator was administered either immediately after delivery or to 4-week-old animals. During physiological maturation of the immune system and the disappearance of...
The aim of the study was to determine the influence of various doses of lipopolysaccharides (LPS) on the phagocytic activity of neutrophils in 12 clinically healthy mares. Three mares were regarded as the controls; the experimental groups consisted of the remaining animals divided into 3 groups of 3 mares each. All experimental animals were treated with intravenous infusion at doses of 0.02, 0.05,...
A study on the seroprevalence of FMD antibodies in cattle in the „ring” vaccination zone was performed. Out of 550 samples of sera examined by LPB ELISA, 240 samples (44%) have been found positive to FMDV type A,O and/or C (ELISA titres > log 1.6). The highest Ievel of antibodies was found for FMDV type O₁BFS; about 10% of tested sera had titres > log 3.01. A half-life of maternal antibodies...
Effect of ketosis on the number and activity of leukocytes was investigated in 8 cows. The high level of ketone bodies in the blood was associated with a significant decrease in the total white blood cell count, neutrophils, and eosinophils, and insignificant decrease in lymphocytes. The number of monocytes was not changed. At the stage of ketosis, the neutrophil ability to NBT reduction was significantly...
The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically...
Analysis of multiple regression of data obtained on 347 donor cows revealed a statistically significant interaction (P<0.001) between FSH drugs and: 1- the age of cows. 2 - month of examination and the number of transferable embryos and month of their yields. 3 – the number of superovulations per cycle and the number of transferable embryos.
The level of glucose, total cholesterol and its HDL fraction, triglycerides, and free fatty acids were determined 4 weeks before delivery and 2 weeks postpartum in 45 cows. The estimation of ovary function was performed by measurements of progesterone in blood sampled every 3 or 4 days during a 60-day postpartum period. A positive correlation was found between disturbances in lipid metabolism and...
Statistical analysis of results indicated a significant positive correlation (r = 0.676, P<0.001) between manganese concentrations in serum and blood of cows. Manganese concentration measurements of 147 serum and 117 blood samples from cows in various stages of their production period and fed various manganese levels in their feed were used for determination of the range of reference values. The...
In the present study field strains of equine herpesvirus 1 (EHV 1) were compared by restriction enzyme analysis. The strains were isolated from aborted fetuses in a horse stud during an abortion outbreak in 1993/1994. We found that all isolates had the same electrophoretic patterns of EHV 1 type, very similar to the pattern of the reference Ab-4 strain of EHV 1. We confirmed also the usefulness of...
The changes in titratable acidity of white cream yoghurt were observed during its ageing and storage dependently on the addition of sodium nitrate and nitrite into the fermenting medium. Dynamics of residual nitrates and nitrites was investigated in parallel. Sodium nitrate was added to the yoghurt mixture, obtained directly from the fermentation tank, in Agromilk Sabinov plant, in an amount so that...
Investigations aimed to evaluate the effectiveness of Boviseptivac, vaccine against bovine pasteurellosis prepared from the Polish isolates of serotype B:2 with an oil adjuvant (Emulsigen). The vaccine protective value obtained on white mice was 4.25 according to the method of Ose and Muenster. Field trials carried out on 120 cows in the Ostroleka province revealed advantageous properties of the vaccine,...
A method for blood manganese measurements has been described. The method provides high reproducibility of the concentrations measured. The coefficients of variation were 3.4% and 4.1 % for within-run and between-runs, respectively. The recovery of the method was 98.1 %. Manganese concentrations in the blood ranged from 6.27 ng/ml to 8.98 ng/ml. The measurements revealed that contamination of tubes...
Monoclonal antibodies (MAbs) specific to avian pneumoviruses were obtained for the diagnosis of turkey rhinotracheitis (TRT) and swollen head syndrome (SHS). The vaccinal BUT strain of TRT virus was used as an antigen for mouse immunisation. The virus was propagated in a Vero cell culture followed by purification and concentration by ultracentrifugation. The obtained antigen was used for threefold...
After intramuscular administration to laying hens, the highest mean concentration of oxytetracycline in (OTC) egg white was noticed on the 1st day after treatment (the 6th day of the experiment). On the 6 th day after treatment OTC was not detected. In the egg yolk, the highest OTC concentration was observed on the 3rd day after treatment. On the 13 th day after treatment no residues of oxytetracycline...
The polymerase chain reaction (PCR) was used to demonstrate the presence of CPV DNA in tissue cultures and faecal suspensions. A simple procedure of sample preparation was elaborated and it made the PCR easily applicable in rapid confirmation of the diagnosis of parvovirus infection. The results of PCR were compared with virus isolation, haemagglutination and immunoenzymatic tests.
This work aimed to assay the physiological values of basie blood coagulation indices and to determine the influence of breed on their range. Studies involved 105 horses of 6 breeds: Arabians, Thoroughbreds, Great Polish, Pony, Polish Primitive Horses and other breeds. It was found that under physiological conditions a mean coagulation time, kaolin-cephalin time, prothrombin time and thrombin time...
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